Which Type Of Blast Search Did You Choose, And Why? Report Examples

Report

Question 1

The unknown sequence is the sequence of nucleotides, so that’s why we have chosen the nucleotide blast search. There are four kinds of nucleotides in the sequence T, A, C, G.

Question 2

What is the name of the gene?
The name of the gene is an acronym of 8 letters that refers to the organism the gene is from.
It is MsFtsZ-mt. The full name is Mallomonas splendens FtsZ mRNA, it is nuclear gene that is encoding mitochondrial protein.

Question 3

What organism has the unknown sequence come from?
The scientific name of the organism is Mallomonas.
What supergroup of eukaryotes does the organism belong to?
According to the taxonomical classification, that divides all living organisms into two biggest groups and various smaller taxonomic groups. It also gives the species name, that the gives the organism a specific name and place in the huge amount of living creatures. The identification of the unknown organism shows its belonging to the Eucaryota and the next sublevels from the Stramenopiles; Synurophyceae; Synurales;Mallomonadaceae to Mallomonas.

Question 4

Write the full reference of the publication (i.e., the paper/manuscript) that first
described the gene and protein that the unknown sequence is part of. Provide the correct standard referencing format that you would see in a reference list from a paper in the Journal of Cell Biology
Beech P., Nheu T., Schultz T., Herbert S., Lithgow T., Gilson P., McFadden G.,2000.Mitochondrial FtsZ in a chromophyte alga. Science. 18, 287, pp. 54-56.

Question 5

How many nucleotides are there in the coding sequence for this protein?
There are four types of nucleotides, different combination of which creates the great variety of the living organisms. Usually genes are coded by the three nucleotides joined together, that are called triplets. In this sequence there are 1215 nucleotides.

Question 6

What is the STOP codon for the gene?
The stop codon is the triplet of nucleotides in the sequence of the messenger RNA, it is a combination of appropriate or specific nucleotides. The key role and function lie in the termination of the translation. There are three types of stop codons: UGA ("opal"), UAG ("amber"), UAA ("ochre") in the RNA and in the DNA they are called TAG ("amber"), TGA ("umber" or "opal"), TAA ("ochre"). In this case it is TGA ("umber" or "opal").

Question 7

What do we call the two sets of sequences that are downstream (3 prime) of the coding sequence?
The gene expression regulates by many inner cellular mechanisms. The unit of the DNA, that is transcripted consists not only of the coding sequence, but also of the regulatory sequences, that control and directs the protein synthesis, this sequence is called 5'UTR - five prime untranslated region. The abbreviation of the coding sequence is 3'UTR or three prime untranslated region( downstream from). Transcriprion of the DNA started from 3' and directs to 5' end. The complementary RNA is made in the 5' → 3' direction.

Question 8

The main function of regulatory sequence is the process of regulation of the protein synthesis on the molecular level, by changing the gene expression.

Question 9

How many amino acids are there in the entire protein that the unknown sequence encodes part of?
There are 401 amino acids in the defining protein, that is coded by the unknown sequence.
Question 10
Give the reason for why the number of nucleotides that code for the protein is not exactly 3 times the number of amino acids that are translated.
The theory about one gene – one amino acid is not correct. The latest researches state, that one amino acid can be coded not only by the one codon, but by the several, that is why we can’t count the number of amino acids only as 1/3 from the number of nucleotides.

Question 11

So let’s describe the main qualities of this gene. This gene is encodes by the putative mitochondrial division protein, that is located in the mitochondria in the cell. This protein play a significant role in the division of mitochondria. (Beech P., 2000). Also, it functions in the division of chloroplast.(Osteryoung K., 2003). Another study observed the action of this protein in the plastid division. (Wang D., 2003) It is also known as a mitochondrial division protein. (Beech P., 2000). One of the research shows that bacteria use the variant of this protein to divide, in the other words the FtsZ protein participate in its division.( Sheng W.,2003).

References

Beech P., Nheu T., Schultz T., Herbert S., Lithgow T., Gilson P., McFadden G.,2000.Mitochondrial FtsZ in a chromophyte alga. Science. 18, 287, pp. 54-56.
Osteryoung K., Nunnari J., 2003. The division of endosymbiotic organelles. Science 302(5651),1698-704.
Sheng W., Hua X., Yu S., Wu W., 2003. Cloning and expression in E.coli of Chlamydomonas reinhardtii CrFtsZ3 gene. 11, pp.998-1004.
Wang D., Kong D., Wang Y., Hu Y., He Y., Sun J., 2003. Isolation of two plastid division ftsZ genes from Chlamydomonas reinhardtii and its evolutionary implication for the role of FtsZ in plastid division. J Exp Bot. 54(384), 1115-6.